| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생체재료 |
| 제목 |
Temporally controlled and color-switchable fluorescence activation in live bacteria |
| 초록 |
Fluorescent labeling of proteins is crucial for bioimaging to study complex molecular mechanisms in cells. The yellow fluorescence-activating and absorption-shifting tag (Y-FAST) is a 14-kd protein, which is capable of temporal control of fluorescence signal by fluorogen binding. It can induce color switching of bacteria or mammalian cells by utilizing reversible reaction. Unlike conventional fluorescence-activatable proteins, Y-FAST is very fast and completely reversible to fluorogen. Various fluorogens were synthesized which were shown to result in photo-activation of Y-FAST in different colors upon their binding to the protein. The fluorogens were shown to reversibly bind to Y-FAST, while sequential addition of various fluorogens resulted in their spectral shifts to different colors. We plan to apply this study as a reporter system for multicolor and temporally controlled imaging of live cells as well as bacteria. |
| 저자 |
김주강, 이해성, 이해신, 정현정
|
| 소속 |
한국과학기술원 |
| 키워드 |
Fluorescence; bioimaging; chemical tag; photoactivation
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| E-Mail |
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