| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Study for the Gramicidin A Activities in the Presence of Ionic liquids (ILs) Using Model Cell Membrane and Simulation |
| 초록 |
As the toxicity of ILs has not been proved in aquatic environment, it cannot assume that ILs are completely safe. Hence, we investigate mechanism of ILs between membrane and membrane protein using model cell membrane in this work. The ion permeability of gA varies depending on the state of the bilayer, we confirmed it using two methods; fluorescence assay utilizing using stop-flow spectrometer and measurement of ion currents across lipid bilayers using a patch clamp instrument. Moreover, we revealed that alkyl chain length of ILs and ion strength of buffer play an important role in the permeability and confirmed how electrostatic effect changed depending on ion strength of buffer using MD simulation. |
| 저자 |
류현일1, 이환규2, 최상백1, 김영록3, 김선민1, 전태준1
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| 소속 |
1인하대, 2단국대, 3경희대 |
| 키워드 |
lionic liquids; lipid bilayer; gramicidin A; toxicity; membrane
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| E-Mail |
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