| 초록 |
Minicells are small sphere-shaped cells formed by aberrant cell division, which are capable of other cellular processes as well as protein synthesis. However, because bacterial minicells have no chromosomal DNA, there are no bacterial growth and cell division. In this study, we genetically engineered ftsZ, minCD and mukB genes related bacterial cell division to increase the formation of minicells in Escherichia coli. Through increased minicell formation, the improved tolerance was exhibited in the mutant strains, which brings about the enhanced production yield about toxic compounds. Furthermore, the function of minicells was not affected in accumulation of toxic compounds because chromosomal DNA is absent in minicells. Based on these characteristics, minicell mutants has great potential as chassis for production of toxic compounds such as isobutanol, isobutylaldehyde, and isobutyl acetate with high production yield. |
