| 초록 |
Lignocellulose is a promising raw material for biochemical production. However, the lignin complex and crystalline cellulose require pretreatment steps for breakdown of the crystalline structure of cellulose for the generation of fermentable sugars containing several fermentation inhibitors such as furfural and acetate. To mitigate these inhibitors, we applied several strategies to overcome these and produce more isobutanol. First, amino acids were investigated on furfural-induced growth inhibition in E. coli producing isobutanol and discovered glycine and serine are the most effective compounds against furfural. Second, we investigated combinatorial application of two oxidoreductases, FucO and YqhD suggesting that combinatorial overexpression of FucO and YqhD could overcome the inhibitory effect of furfural giving higher isobutanol. Third, nicotinamide salvage pathway genes were overexpressed resulting in increasing the tolerance of the cells to toxic aldehydes. |
