| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 봄 (04/07 ~ 04/09, 대전컨벤션센터) |
| 권호 |
46권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 Ⅰ |
| 제목 |
Cell Spheroid Culture System with Porous Microparticle for Prolonged Cell Survival and Enhanced Osteogenic Differentiation |
| 초록 |
Development of three-dimensional (3D) cell spheroid systems is considered as a promising technology to mimic in vivo microenvironment compared to monolayer-culture. However, limited size of spheroid due to the insufficient supply of oxygen/nutrients into the central region is still remained as a critical huddle. In this study, we developed microparticles with leaf-stacked structure (LSS particles) which can allow sustained release of bioactive molecules as well as provide space for sufficient supply of oxygen/nutrients. The cell spheroid was prepared using the LSS particles and human bone marrow-derived mesenchymal stem cells (hBMSCs). The morphology of LSS particles and cell/LSS spheroid was observed by a scanning electron microscope (SEM) and release behavior of BMP-2 from LSS particles was determined using a ELISA kit. Cell survival/structural stability/osteogenic differentiation (in vitro) and new bone formation (in vivo) of cell/LSS spheroid system were investigated. |
| 저자 |
김민지1, 정영조2, 이진호2, 오세행1
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| 소속 |
1단국대, 2한남대 |
| 키워드 |
Cell spheroid; Microparticles; Bone morphogenetic protein-2 (BMP-2); human bone marrow-derived mesenchymal stem cells (hBMSCs); Bone regeneration
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| E-Mail |
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