| 초록 |
We herein describe a rapid detection method for protein/small molecule (SM) interactions based on simple principles of protein-induced fluorescence enhancement (PIFE). Especially, ligand-receptor interaction is detected by measuring fluorescence signal induced by close distance by optimization. When a target ligand combined with aptamer-Cy3 binds with receptor, it comes to the vicinity of Cy3, leading to PIFE on Cy3. As a result, the significant fluorescence enhancement is observed from Cy3. With this approach, we successfully detected a model target interaction, thyroid hormone/receptor interaction, within 10 min. Also, various inhibitors was screened, verifying its applicability. Finally, the practical applicability of this method was demonstrated by reliably determining thyroid hormone in human serum. |
