| 학회 |
한국공업화학회 |
| 학술대회 |
2005년 가을 (10/28 ~ 10/29, 건국대학교) |
| 권호 |
9권 2호 |
| 발표분야 |
정밀화학 |
| 제목 |
Fluorescence Analysis of Protoporphyrin IX induced from 5-aminolevulinic acid in incubated HeLa cell and normal liver cell |
| 초록 |
To clarify the usefulness of fluorescent diagnosis for lung cancer, we investigated the optimal method of administrating 5-aminolevulinic acid (5-ALA) by analyzing fluorescence signal of Protoporphyrin IX (PpIX) in the cultured cancer and normal liver cells. 5-ALA was injected as a photosensitizer to the cervico-uterine cancer cell line (HeLa) and in normal liver cells (Chang). Chang and HeLa cells were incubated with various concentrations of 5-ALA (0-800 ㎍/㎖). The accumulation of PpIX induced by 5-ALA was in HeLa and Chang cells. The optimal concentration of ALA that induced maximum levels of PpIX was 200 ㎍/㎖ in HeLa cell cured for 24 hours after 5-ALA injection. The experimental results showed that fluorescence signal of PpIX was proportional to the concentration of 5-ALA in tumor cells, but not measured in normal cells. |
| 저자 |
김명화, 도영국, 김경찬, 이인선, 이창섭
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| 소속 |
계명대 |
| 키워드 |
Fluorescence Analysis; 5-aminolevulinic acid; Protoporphyrin IX; HeLa cell
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| E-Mail |
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