| 초록 |
In this study, the surface of mesenchymal stem cells (MSCs) was coated with multilayered nanofilm to promote chondrogenesis by using fibronectin (FN) and chondroitin sulfate (CS). Our result showed that chondrocyte marker expression and cell proliferation depend on the number of nanoshell layers. We regarded that the thickness of the nanofilm that is adsorbed on the cell surface is related to the duration time of the effect occurred by the binding of cell membrane and FN, CS. At the same time, the nanofilm also showed a negative effect of inhibiting growth by isolating cells from the outside. Thus, precisely controlling the structure of the nanofilms deposited on the cell surface is essential for promoting cartilage differentiation. So, we also analyzed the effect of nanofilms on the cell surface on the physical and biochemical aspects and we investigated changes in chondrocyte marker expression over time to found the mechanism of the nanofilm effect. |
