| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 봄 (04/25 ~ 04/27, 창원컨벤션센터) |
| 권호 |
24권 1호, p.207 |
| 발표분야 |
공정시스템 |
| 제목 |
Anodic Electro fermetation Facilitated Biochemical production by P. putida 2523 in Microbial fuel cell |
| 초록 |
Pseudomonas putida strain has been highlighted as a host cell for microbial production of a range of amphiphilic and aromatic biochemical. P. putida is also known as electro-active in a microbial fuel cell system to produce electricity, which indicates that electron transportation via outer membrane is feasible. Thus this strain is an ideal host for metabolic engineering for platform chemical production using electro based metabolic regulation. In this study, we have examined the metabolic shift of P. putida in different culture condition such as conventional fermentation and electrode respiring environment. The cell growth of P. putida was facilitated by electrode with simultaneous electricity generation and changes of by-product formation. This strain during electro-fermentation discharged current up to 500A/cm2 anodic current density and few metabolites acetate, lactate. Metabolite concentrations and glucose concentration were measured by using HPLC. These results implicate that metabolically engineered P. putida can provide a platform for BES based bio refinery process with further metabolic engineering and optimization. |
| 저자 |
Sakuntala Mutyala, 김중래, 김창만, 송영은
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| 소속 |
부산대 |
| 키워드 |
화학 및 생물공정 |
| E-Mail |
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| 원문파일 |
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