| 초록 |
The microflow together with microfluidics exists at the intersection of engineering, physics, chemistry, and biotechnology, spanning scientific and technological developments. Micro/nano technologies play an enabling role to improve the smart healthcare which becomes an escalating challenge on a range of translational, personalized medicine, and diagnostics. This invited talk will bring our studies on microfluidic-chip based cell/particle sorting and pathogen detection. We performed continuous sorting of human mesenchymal stem cells (hMSCs) by using optimally designed microfluidic chips based on the hydrodynamic filtration (HDF) principle. To further improve sorting efficiency and throughput, a spiral channel in the upper layer designed for inertial focusing by Dean flow was coupled with the lower HDF layer. Sorting efficiency has been compared to the result obtained by the FACS, and recovery and purity were achieved higher than those of previous results. Pathogenic E. coli detection has also been investigated by applying a microfluidic based biosensing device labeled with antimicrobial peptides onto channel surface, with detection limit of 10 cells/mL in suitable flow condition. |
