| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 봄 (04/10 ~ 04/12, 부산컨벤션센터(BEXCO)) |
| 권호 |
44권 1호 |
| 발표분야 |
대학원생 구두발표 (영어발표, 발표15분) |
| 제목 |
Harmless retrieval of functionally-enhanced spheroids from thermoresponsive microwell for further in vivo applications |
| 초록 |
In this study, we have designed fibrous and thermoresponsive microwell scaffold, so the cellular spheroids cultured on the scaffold can be harmlessly retrieved through temperature changes. The cellular spheroids are known to have enhanced functionality since the 3D structure of cellular aggregates closely mimic the organ structures. Therefore, instead of implanting single cells to the injured site of tissues, the spheroids would result in better consequences for regeneration of damaged tissues. The thermoresponsive characteristics are evoked from the usage of poly(N-isopropylacrylamide (pNIPAAm), which has a LCST (lower critical solution temperature) of 32℃. The hydrophobic property at above the LCST will allow cellular adhesion, and the hydrophilic property at below the LCST will allow cellular detachment, finally letting the spheroids to be retrieved innocuously. The collected spheroids then can further be used in various applications for efficient tissue regeneration. |
| 저자 |
홍혜진, 윤병주, 김세민, 김혜원, 신현수, 노윤진, 임재열, 고원건
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| 소속 |
연세대 |
| 키워드 |
3D culture; microwell; spheroid; salivary gland stem cell
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| E-Mail |
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