| 초록 |
Through controlled release of growth factors by multi-layered scaffolds, regeneration of sciatic nerves has been promoted. The scaffold consists of three layers; PCL aligned, PLGA 6535 random nanofibers, and random fibers of PLGA 8515. The PLGA layers act as reservoirs for release of growth factors; NT-3, BDNF, and PDGF. In rat model, the injured nerve, which was rolled up by the growth factors laden and multi-layered scaffold, was recovered after 5 weeks from the time of surgery. The initially released NT-3 and BDNF from PLGA 6535 have especially promoted regeneration of the neural tissue, and the sustainably released PDGF from PLGA 8515 further enhanced the regeneration procedure to replace the function of hind limb locomotor activity. This study demonstrated that the multi-layered scaffold has performed a function that can be used to enhance nerve regeneration in combination with aligned topography of fibrous scaffold and controlled release of growth factors. |
