| 초록 |
Colorimetric biosensors have received wide attention due to their simple operation, low cost and rapid detection. However, relatively low sensitivity limits their applications. Herein, we developed a sensitive colorimetric biosensor for detecting A. niger spore using phage display-identified spore-binding phages that were biotinylated for binding with streptavidin-conjugated HRP. The phages were biotinylated on numerous p8 capsid proteins, which enables signal enhancement through biotin/streptavidin-HRP interactions. Next, we performed flow cytometry assay to compare the binding affinity of identified phages toward the spore. This sensor phage could effectively distinguish A. niger spore from other control microorganisms and showed a good sensitivity. These results show that the engineered phage indicates an attractive candidate with signal enhancement capability for sensor application. This strategy can be applied to sensing various targets by altering phage sequence. |
