| 초록 |
Photosynthetic organisms evolved phototaxis to find optimal light condition for photosynthesis. Here we report a microfluidic screening using competitive phototaxis of Chlamydomonas reinhardtii, for rapid isolation of strains with improved photosynthetic efficiencies. We demonstrated correlation between phototaxis and photosynthetic efficiency by quantitative analysis of phototaxis using a microfluidic system. We enriched the strains with improved photosynthetic efficiency by isolating cells showing fast phototaxis from 10,000 mutants, thereby greatly improving selection efficiency over 8 fold. Among 147 strains isolated after screening, 94.6% showed improved growth over the parental strain. Two mutants showed much improved performances with up to 1.9- and 8.1-fold increases in growth and lipid production, respectively. Our approach provides a powerful screening tool for rapid improvement of microalge to enhance photosynthetic productivity. |
