| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터) |
| 권호 |
43권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Gene Delivery of PAMAM Dendrimer Conjugated with KRTR peptides originated from Influenza B Virus Nucleoprotein |
| 초록 |
The polyamidoamine (PAMAM) dendrimer is one of the cationic polymers that are widely used in nonviral gene therapy research, and PAMAM shows a sufficient biocompatibility and relatively low cytotoxicity. it has poor transfection efficiency In comparison to polyethylenimine (PEI, 25 kDa). To improve transfection efficiency, we introduced the KRTR peptide derived from influenza B virus nucleoprotein into PAMAM. We synthesized PAMAM-RTRK and PAMAM-HRTRK to determine the difference in their cytotoxicity. PAMAM-RTRK and PAMAM-HRTRK showed a transfection efficiency that corresponded to that of PEI in HepG2 and A549 cell lines. These results imply that the combination of histidine with the KRTR peptide could potentially provide a novel PAMAM-based vector that could be used prolonged gene therapy by reducing cytotoxicity and enhancing gene expression. |
| 저자 |
이제일, 최준식
|
| 소속 |
충남대 |
| 키워드 |
cationic polymer; polyamidoamine dendrimer; influenza B virus; nuclear localization
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| E-Mail |
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