| 초록 |
Galactose and glucose can be achieved by dilute acid or enzymatic treatment of red seaweed, many bio-chemicals can be produced from these sugars through fermentation process. However, the challenging problem is that galactose is not favored by many industrial microorganisms. Slow utilization rate and carbon catabolite repression limits carbon flux and results in low productivity and titer. In this research, we introduced two synthetic modules to utilize sugars from hydrolysate of red seaweed (Gelidium amansii) and to produce n-butanol, one of advanced fuel. The modules with metabolic enzymes under synthetic promoters, rationally designed 5’UTRs (5’untranslated region), and terminators were assembled, and these modules were optimized by finding maximum productivity with various expression of specific gene. With this approach, the engineered strain showed significantly enhanced sugar utilization and n-butanol production from both red seaweed mimetic media and real hydrolysate. |
