| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 봄 (04/09 ~ 04/10, 고려대학교) |
| 권호 | 29권 1호, p.179 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Neuronal differentiation of BMSCs in BME/PLGA scaffold |
| 초록 | Current tissue engineering techniques require the use of a porous biodegradable/bioresorbable scaffold, which serves as three-dimensional template for initial cell attachment and subsequent tissue formation, both in vitro and in vivo. Bone marrow stromal cells (BMSCs) can be differentiated in culture into osteoblasts, chondrocytes, and even neurons. BMSCs can be differentiated to neuronal phenotype in supplement medium with β-mercaptoethanol (BME). Porous PLGA scaffolds slowly releasing BME were prepared by means of ice particle leaching methods. Characterizations of BME contained PLGA scaffold were analyzed by SEM, mercury porosimeter, release profile and so on. Cellular viability and proliferation were assayed by MTT test. Neurogenic differential cell were analyzed by immunohistochemical staining for NSE and NF. In conclusion, we observed that BMSCs were differentiated to neuronal phenotype and BME was slowly released from PLGA scaffold. Acknowledgment This work was supported by grant form KRF |
| 저자 | 장우영1, 장지욱2, 김은정1, 이일우3, 김문석2, 조선행2, 이해방2, 강길선1 |
| 소속 | 1전북대, 2한국화학(연), 3카톨릭의과대 |
| 키워드 | tissue engineerin; scaffold; PLGA |
