| 초록 |
Pseudomonas putida has been drawn much attention as a bacterial host for synthetic biology, metabolic engineering, and biocatalysts due to its safety status, rapid growth, low nutritional demand, and considerable resistance to inhibitory chemicals. Here, we applied this bacteria as a host strain for surface display of trehalose synthase, which is derived from the Pseudomonas putida KT2440 strain. By expressing treS on the cell surface, it can be used as an immobilized biocatalyst converting inexpensive and economical sugar into high-value trehalose. Surface display of trehalose was confirmed by Western blot analysis, immunofluorescence microscopy, and measurement of whole cell activity. To achieve a high conversion rate through expression of trehalose synthase on the P. putida surface, two anchoring motifs were employed and various reaction conditions were examined. |
