| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.1229 |
| 발표분야 |
[주제 12] 화학공학일반(부문위원회 발표) |
| 제목 |
Cell surface display of Pyrococcus horikoshii glutamate decarboxylase in Escherichia coli for high-titer extracellular gamma-aminobutyric acid production |
| 초록 |
The effect of cell-surface display of glutamate decarboxylase (GadB) on gamma-aminobutyric acid (GABA) production in recombinant Escherichia coli was investigated in this study. We integrated GadB from hyperthermophilic, anaerobic archaeon Pyrococcus horikoshii to the C-terminus of the E. coli outer membrane protein C (OmpC). After 12 h of culturing GadB displaying cells, the GABA concentration in the extracellular medium increased to 3.2 g/l, which is eight times that obtained with cells expressing GadB in the cytosol. To further enhance GABA production, we increased the temperatures of the culture. At 60°C, the obtained GABA concentration was 4.62 g/l after 12 h of culture, and 5.35 g/l after 24 h, which corresponds to a yield of 87.7%. |
| 저자 |
Kumaravel Ashokkumar1, 정재훈2, 홍순호2
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| 소속 |
1Univ. of Ulsan, 2울산대 |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
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