| 학회 |
한국고분자학회 |
| 학술대회 |
2022년 봄 (04/06 ~ 04/08, 대전컨벤션센터) |
| 권호 |
47권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Synthesis and characterization of polyamidoamine dendrimers conjugated with cholesteryl-dipeptide as gene carriers |
| 초록 |
PAMAM dendrimers have been widely utilized because of the abundance of terminal functional groups, thereby enabling further functionalization and enhancing DNA condensation and internalization into cells. Herein, we synthesized PAMAM G2-HRChol by conjugating dipeptide (HR) and cholesterol at different ratios (6% or 23%) on the surface of PAMAM G2. The transfection efficiency of PAMAM G2-HRChol 6% and PAMAM G2-HRChol 23% dendrimers was similar to that of PEI 25 kDa under optimum conditions, and the cytotoxicity was much lower than that of PEI 25 kDa in HeLa cells. In addition, after apoptin gene transfection was performed, cell death ratios of 34.47% and 22.47% were observed for PAMAM G2-HRChol 6% and PAMAM G2HRChol 23%, respectively. The results show that a suitable amount of cholesterol can improve gene transfection efficiency, and the PAMAM G2-HRChol 6% dendrimer could be a potential gene carrier in HeLa cells. |
| 저자 |
래티튀1, 최민영1, Minhyung Lee2, 최준식3
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| 소속 |
1충남대, 2Department of Bioengineering, 3College of Engineering |
| 키워드 |
PAMAM dendrimer; cholesterol; dipeptide; gene delivery; transfection
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| E-Mail |
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