| 초록 |
For successfulgene therapy, it is significant to deliver gene to target sites without cellularelimination and minimizing off-target effects in vivo. In this study, weutilized human mesenchymal stem cells (hMSC) as a carrier for reducing damage tonormal tissues and achieving local treatment by secreting cytokine. We used cationicpolymer (CP), non-viral vector, complexed with plasmid DNA (pC) expressing tumorcell apoptosis-inducible cytokine. Photochemical internalization (PCI) was usedto enhance transfection efficiency of non-viral vector. We verified that when pC-CPwas internalized into hMSC via PCI (pC-CP@hMSC_PCI), cellular internalization andcytokine expression augmented compared to unloaded pC-CP groups. Furthermore, weconfirmed that therapeutic ability by the homing effect and anti-tumor effect ofpC-CP@hMSC_PCI in mouse model of tumor. Based on the results, we confirmed thatpC-CP@hMSC_PCI can be a novel therapeutic agent for stem cell-mediated cancer genetherapy. |
