| 학회 |
한국공업화학회 |
| 학술대회 |
2010년 가을 (10/27 ~ 10/29, 대전컨벤션센터) |
| 권호 |
14권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Multiple sequence alignment-assisted mutagenesis of marine epoxide hydrolase |
| 초록 |
The marine fish microsomal epoxide hydrolase (mEH) was mutated to increase the hydrolytic activity toward racemic epoxides by using multiple sequence alignment. The amino acid sequences of Aspergillus niger, Rhodotorula glutinis, zebra fish and human mEH were aligned and analyzed for their difference. Three types of single-point mutants were developed and compared. Chiral (S)-styrene oxide could be readily prepared with high enantiopurity more than 99%ee by using the MSA-assisted mutant. |
| 저자 |
신정원1, 최성희2, 김희숙2, 이은열1
|
| 소속 |
1경희대, 2경성대 |
| 키워드 |
mutagenesis; (S)-styrene oxide; epoxide hydrolase
|
| E-Mail |
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