| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 가을 (10/22 ~ 10/23, 일산 KINTEX) |
| 권호 | 15권 2호, p.1634 |
| 발표분야 | 생물화공 |
| 제목 | Biotransformation of Chrysin into Baicalein through ortho-hydroxylationusing Streptomyces Ceolicolor |
| 초록 | Chrysin (5,7-dihydroxyflavone) is a naturally occurring flavone chemically extracted from the blue passion flower. Dietary flavonoids have many biological properties that could make them useful as chemopreventive agents. However, very poor oral bioavailability makes them largely ineffective in vivo. In previous studies, hydroxylated flavonoid compounds are certificated more effctive absorption in vivo than flavonoid compounds. We selected the chrysin as substrate. Streptomyces Ceolicolor showed high ortho-hydroxylation activity to produce baicalein (5,6,7-tetrahydroxyflavone). Streptomyces Ceolicolor incubation condition is 28℃, 220rpm for 4 day (96h), using R2YE media 100ml. We were reacted using PBS (Phosphate buffered saline) buffer 100ml, chrysin 10mM 2ml, incubated cell for 4h. The reaction product was analyzed using HPLC and GC/MS. Time-course and conversion of substrate will be discussed. P450 inhibitor assay and reaction profile with each detergent will be also discussed. |
| 저자 | 노지원1, 김준형1, 김병기2 |
| 소속 | 1동아대, 2서울대 |
| 키워드 | flavonoids; chrysin; biotransformation; streptomyces ceolicolor |
| 원문파일 | 초록 보기 |
