| 초록 |
Compared with synthetic scaffold, biopolymeric scaffold has been considered more profitable to regulate adhesion, differentiation and migration of cells. Alginate is biocompatible, nonimmunogenic and rapidly forms a gel by adding multivalent cations. To improve cell-interactive properties of alginate hydrogel, proteins including gelatin are often included. Gelatin is a biopolymer obtained after hydrolysis of collagen which is the major protein of the ECM. Gelatin has low immunogenicity and excellent bioaffinity. In this study, we fabricated alginate/gelatin hydrogel by dual crosslinking in order to retain more gelatin in the hydrogel. To obtain optimum condition of hydrogel, we regulated the ratio of alginate to gelatin methacrylate. Gelatin content in the hydrogel was calculated by measuring the solid content with a moisture analyzer. The structure of hydrogel was investigated by 1H-NMR, FT-IR and SEM. In addition, we evaluated mechanical properties of hydrogel by UTM and rheometer. |
