| 초록 |
We demonstrate the preparation of bicontinuous nanoporous covalent frameworks which are promising for caging active enzymes. The frameworks have three-dimensionally continuous, hydrophilic pores with width varying between 5 and 30 nm. Enzymes were infiltrated into the bicontinuous pore by applying a pressured enzyme solution. The new materials systems allowed the amount of caged proteins to be controlled precisely. The resulting enzyme-loaded framework films could be recycled many times with nearly no loss of catalytic activity. Entropic trapping of proteins by a bicontinuous pore with right size and size distribution is an unprecedented strategy toward facile in-vitro utilization of biocatalysts. |
