| 초록 |
Peptides with the sequence of RGD and YIGSR have been frequently used to regulate many critical aspects of cell adhesion, proliferation, and differentiation. The RGD, present in major ECM proteins, has been intensively investigated as a cell-binding sequence, and the YIGSR, derived from the laminin B1 chain, interacts with the laminin binding protein that can be found in neurons and involved in neuronal differentiation, like neurite outgrowth. In this study, we modified alginate hydrogels with both RGD and YIGSR peptides at various densities, and investigated how PC12 cells responded to these dual peptide-modified gels. The RGD peptide significantly enhanced cell adhesion as expected, and the YIGSR peptide was critical in differentiation of PC12 cells. In particular, the presence of both RGD and YIGSR peptides showed the synergistic effect for differentiation of PC12 cells. Hydrogels modified with multiple adhesion peptides could find useful applications in tissue engineering. |
