| 학회 |
한국화학공학회 |
| 학술대회 |
2009년 가을 (10/22 ~ 10/23, 일산 KINTEX) |
| 권호 |
15권 2호, p.2113 |
| 발표분야 |
재료 |
| 제목 |
Colorimetric detection of mutation in DNA sequences using selective aggregation of gold nanoparticles |
| 초록 |
We have detected mutations in the epidermal growth factor receptor (EGFR) of non-small cell lung cancer patients using the selective aggregations of gold nanoparticles. Mutations in exon19 and exon21 of EGFR gene were detected in non-amplified genomic DNAs isolated from both the lung cancer cell lines and the cancer tissues of non-small cell lung cancer patients. At the optimal salt concentration, addition of the mutant DNA that was hybridized with the complementary probe into the suspension of unmodified gold nanoparticles caused the substantial aggregation the gold nanoparticles and the color change of solution. Gold nanoparticles, however, did not exhibit significant aggregation and the solution color remained unchanged with the addition of the wild type DNA hybridized with the probe. We could detect the deletion mutant form in exon19 and the point mutation in exon21 mutations by selective aggregation with gold nanoparticles. These results were also confirmed by the direct sequencing method. |
| 저자 |
이호섭1, 강태경1, 주상우2, 이소영3, 윤경아4, 이강택1
|
| 소속 |
1연세대, 2숭실대, 3서울대, 4국립암센터 호발암연구부 |
| 키워드 |
EGFR; Colorimetric; gold nanoparticles; aggregation
|
| E-Mail |
|
| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
