| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 봄 (04/08 ~ 04/09, 대전컨벤션센터) |
| 권호 |
35권 1호 |
| 발표분야 |
세포기능조절 고분자(의료용 고분자 부문위원회) |
| 제목 |
Porous Poly(lactic-co-glycolic acid) Microsphere as Both Cell Culture Substrate and Cell Transplantation Vehicle for Tissue Engineering |
| 초록 |
Tissue engineering often requires ex vivo cell expansion to obtain a large number of transplantable cells. However, thetrypsinization process used to harvest ex vivo expanded cells for transplantation interrupts interactions between cultured cells and their extracellular matrices, facilitating apoptosis and consequently limiting the therapeutic efficacy of the transplanted cells. In this study, open macroporous poly(lactic-co-glycolic acid) (PLGA) microspheres were utilized both as a cell culture substrate to expand cells ex vivo and as a cell transplantation vehicle for tissue engineering, thus avoiding thetrypsinization necessary for transplantation of ex vivo expanded cells. Porous poly(lactic-co-glycolic acid) (PLGA) microspheres have been also proposed as an injectable scaffold for in vivo bone and cartilage tissue engineering. In this study, we tested whether using open macroporous PLGA microspheres as an injectable scaffold for in vivo cartilage tissue engineering provides a larger surface area for cell adhesion and a larger void space for bone and cartilage tissue regeneration and thus regeneration of higher quality cartilage than non-porous PLGA microspheres. Additionally, porous poly(lactic acid-co-glycolic acid) (PLGA) microsphere was used both as a cancer cell culture substrate to expand cells and as a cancer cells transplantation vehicle for tumor model construction. Ex vivo cell expansion and transplantation using this system would improve the therapeutic efficacy of cells over the current methods used for tissue engineering. |
| 저자 |
강선웅1, 배유한2, 김병수3, 이수홍4
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| 소속 |
1Department of Biomedical Science, 2CHA Univ., 3Department of Pharmaceutics and Pharmaceutical Chemistry, 4Univ. of Utah |
| 키워드 |
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| E-Mail |
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