| 초록 |
Nucleic acid amplification techniques are used for molecular biology research and medical diagnostics to specifically detect and analyze small amounts of genetic targets. Isothermal amplification has a great advantage over conventional PCR, since the procedure can be performed in simple, miniaturized settings more suitable for point-of-care testing. Herein, we designed a simple, easy and rapid assay based on loop-mediated isothermal amplification (LAMP) of nucleic acid targets and their subsequent hybridization with nanoparticle probes that induce a color change that can be observed with the naked eye. We performed the LAMP reaction for the antibiotic resistance gene KPC, followed by addition with gold nanoparticle probes specific to the loop region of the amplified DNA products. Color changes of the final products were characterized by visualization as well as spectral quantitative analyses. |
