| 초록 |
Raft-based heterogeneity is an important feature of cell membrane because it relates to fundamental biological processes such as signal transduction, membrane trafficking, and endocytosis. To understand the specific role of the lipid raft in the cell membrane, the phase separation phenomenon in the membrane should be scrutinized depending on membrane tension, lipid composition, charge density, temperature and so on. In this work, we developed a new and simple method to make freestanding and planar lipid bilayer model for direct visualization and analysis of phase behavior. Interestingly, compared to other lipid bilayer models such as giant unilamellar vesicles and black lipid membrane, we found that our model had different phase behaviors including domain size and shape, which were significantly affected by geometric shape, interfacial tension, lipid composition, and temperature. Finally, we developed its thermodynamic model using Landau theory on the basis of experimental results. |
