| 학회 |
한국공업화학회 |
| 학술대회 |
2014년 가을 (11/12 ~ 11/14, 엑스코(대구)) |
| 권호 |
18권 2호 |
| 발표분야 |
나노-포스터 |
| 제목 |
Hybrid hyaluronic acid/calcium phosphate gene delivery system enhanced transfection of human stem cells |
| 초록 |
Human mesenchymal stem cells (hMSCs) have shown enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited since there is no appropriate way to control their behaviors in vivo such as differentiation to a desired cell type. Genetic modification of hMSCs may provide an opportunity of controlling behaviors of the cells in an active manner. One of the major huddles for genetic manipulation of hMSCs would be lack of efficient and safe gene delivery system. Herein, an organic-inorganic hybrid gene delivery system based on calcium phosphate (CAP) nanoparticles stabilized with catechol-derivatized hyaluronic acid (dopa-HA) conjugate. The dopa-HA/CAP nanoparticles showed significantly higher transfection in hMSCs than PEI 25kDa. In addition, delivery of BMP2 plasmid DNA with the dopa-HA/CAP formulation achieved desired level of osteogenic differentiation of hMSCs. |
| 저자 |
이정은1, 이민상1, 조용우2, 조현철3, 정지훈1
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| 소속 |
1성균관대, 2한양대, 3보라매병원 |
| 키워드 |
calcium phosphate; mesenchymal stem cells; differentiation; gene delivery
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| E-Mail |
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