| 학회 |
한국고분자학회 |
| 학술대회 |
2002년 가을 (10/11 ~ 10/12, 군산대학교) |
| 권호 |
27권 2호, p.326 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Receptor-mediated delivery of all-trans retinoic acid(RA) to HepG2 from galactosyl α-cyclodextrin(Gal α-CD)/RA inclusion complex |
| 초록 |
Cyclodextrins(CDs) are cyclic (α-1,4)-linked oligosaccharides of α-D-glucopyranose containing a relatively hydrophobic central cavity and hydrophilic outer surface. They are frequently regarded as a new group of pharmaceutical excipients. Intercellular recognition events are fundamental to many biological processes in which oligosaccharides on cell surface glycoproteins or lectins have been responsible for cell-cell recognition and adhesion, etc.. The galactose-substitued CD derivatives may offer a new way of delivering drugs selectively to specific cell surfaces of HepG2. Retinoic acid, the principal active metabolite of vitamin A, is an essential regulator of pattern formation during embryonic development and is necessary for the maintenance of epithelial tissue in the adult. The nature of RA catabolism was clarified with the cloning of a novel gene, CYP26, which encodes a cytochrome P450-related hydroxylase. The metabolism of RA is initiated by hydroxylation mediated by cytochrome P450 activity. The inclusion complex between Gal α-CD and RA was checked by 1H-NMR, X-ray analysis, IR, and differential scanning calorimetry(DSC). Water solubility of RA in Gal α CD/RA was studied. Gene expression of P450RAI(CYP26) between RA itself and Gal α CD/RA was compared by RT-PCR and a modified luciferase system. |
| 저자 |
서석진1, T. Sasagawa*2, S. H. Kim*3, T. Akaike*4, K. Hamayasu**3, 최윤재, 조종수
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| 소속 |
1서울대, 2*Tokyo Institute of Technology, 3Japan, 4**Bio Research Corporation of Yokohama |
| 키워드 |
galactose; cycledextrin; all-trans retinoic acid; P450RAI; luciferase
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| E-Mail |
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