| 초록 |
Adhesion ligand-modified polymers greatly influence the adhesion and proliferation of many cell types, including stem cells. The spacer arm length of the adhesion ligands also play a critical role in controlling multiple aspects of cell phenotype. In our previous study, the spacer arm length of RGD peptides strongly affected the spreading and growth of human primary fibroblasts in both 2-D and 3-D cultures. In this study, we hypothesized that the regulation of spacer arm length of adhesion ligands could direct the stem cell phenotype. RGD-modified alginate promoted the proliferation of D1 stem cells. The growth rate of D1 cells increased as the spacer arm length of RGD peptides increased. The increased spacer arm length of RGD peptides also promoted osteogenic differentiation of D1 stem cells, which was confirmed by analysis of ALP activity. |
