| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 |
16권 1호, p.364 |
| 발표분야 |
생물화공 |
| 제목 |
Environmental Factors Affecting Indole Production in Escherichia coli |
| 초록 |
A variety of both Gram-positive and Gram-negative bacteria produce the intercellular signal indole in microbial communities. While biosynthesis of indole has been well-studied in Escherichia coli, environmental factors affecting indole production for this strain are less clear. This study demonstrates that the environmental cue pH is an important factor for indole production that further controls biofilm formation of E. coli. Moreover, E. coli produced a higher level of extracellular indole in the presence of the antibiotics, and the increased indole enhanced cell survival during antibiotic stress. In a mixed culture of E. coli and Pseudomonas aeruginosa, only a high proportion (above 95%) of P. aeruginosa could significantly degrade environmental indole. Additionally, we found that temperature is another important factor for indole production; E. coli produces and accumulates a large amount of indole at 50ºC, even at low cell densities. Overall, our results suggest that indole is a stable biological compound, and E. coli may utilize indole to protect itself against other microorganisms. |
| 저자 |
한티힙1, 이진태1, 이진형1, 조무환1, Thomas K. Wood2
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| 소속 |
1영남대, 2Department of Chemical Engineering |
| 키워드 |
indole; signal molecule; antibiotics; environmental factors; Escherichia coli
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| E-Mail |
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| 원문파일 |
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