| 초록 |
The antimicrobial peptides P5 and several conventional antibiotics were applied to biofilm-forming clinical isolates of Pseudomonas aeruginosa for susceptibility testing. All of the strains were found to be resistant to the tested compounds. Nonetheless, P5 inhibited the formation of biofilms at very low concentrations and also showed positive synergy when applied at its MBIC50 concentration in combination with vancomycin. P5 also inhibited production of such extracellular matrix components as exopolysaccharides, proteins and nucleic acids, as evidenced by epifluorescence microscopic and spectrofluorometric analyses. The results obtained using this multiparametric approach suggest that P5 could potentially be used as an agent for the prevention of biofilm formation by drug-resistant strains. This study was supported by IPET(Korea Institute of planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries) Research Fund in 2009. |
