| 학회 |
한국화학공학회 |
| 학술대회 |
2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 |
10권 2호, p.1527 |
| 발표분야 |
생물화공 |
| 제목 |
Bacterial Cell Arrays for Environmental Toxicity Analysis |
| 초록 |
In this study, whole cell based cell arrays were fabricated in a chip and 384-well plate by using immobilization technique and recombinant bioluminescent bacteria to detect and classify environmental toxicity. About 2ul of the cell-agar mixture was deposited into the wells of either a cell chip or a 384-well plate. Twenty strains having different stress promoters were used to detect toxicity. Bioluminescence (BL) from the cell arrays was measured with a highly sensitive cooled CCD camera that measures the BL signal from the immobilized cells and quantifies the pixel density using image analysis software. Three chemicals that cause superoxide damage (paraquat), DNA damage (mitomycin C) or protein/membrane damage (salicylic acid) were tested with the cell arrays and depending on the promoter fused upstream of the lux operon, each immobilized strain showed a specific BL response. These arrays could be employed for high-throughput environmental toxicity analysis and used as portable and disposable environmental biosensors due to its small size, ease handling, simple analysis and low cost. |
| 저자 |
이진형, Robert J. Mitchell, 김병찬, 안주명, 구만복
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| 소속 |
광주과학기술원 환경공학과 |
| 키워드 |
Cell chip; environmental biosensor; 384 well plate; bioluminescent bacteria; toxicity analysis
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
