| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Controlled Release of Bovine Serum Albumin from Hydroxyapatite Encapsulated within Hyaluronic Acid Hydrogels |
| 초록 |
A novel sustained release formulation of bovine serum albumin (BSA) was developed using hydroxyapatite (HAp) and hyaluronic acid (HA) hydrogels. BSA was complexed with hydroxyapatite (HAp) through electrostatic interaction between Ca2+ of the HAp and COO- of BSA. For the preparation of HA hydrogels, adipic acid dihydrazide grafted HA (HA-ADH) was synthesized and analyzed with 1H NMR. The degree of HA-ADH modification was ca. 69%. BSA/HAp complex was in situ encapsulated into HA-ADH hydrogels using bis(sulfosuccinimidyl) suberate (BS3) as a selective crosslinker. The denaturation of BSA during HA-ADH hydrogel synthesis was drastically reduced with decreasing pH from 7.4 to 4.8. The specific reactivity of BS3 to hydrazide at pH = 4.8 might be due to its low pKa (pKa = 3.0) compared with that (pKa ＞ 9) of amine of BSA. In vitro release of BSA in PBS at 37℃ could be controlled from two days over a week and will be discussed in detail. |
| 저자 |
염준석1, 한세광2
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| 소속 |
1포항공대, 2포항공과대 |
| 키워드 |
Controlled release; Bovine Serum Albumin; Hyaluronic acid; Hydroxyapatite; Crosslinking; Hydrogel
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| E-Mail |
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