화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터)
권호 20권 1호, p.403
발표분야 생물화공
제목 Metabolic Engineering of Solventogenic Clostridium acetobutylicum for Enhanced Production of Butyric Acid
초록 Clostridium acetobutylicum has been considered an attractive platform-host for biorefinery due to its metabolic diversity. Considering its capability to overproduce butanol through butyrate, it was thought that butyric acid can also be efficiently produced by this bacterium through metabolic engineering. The pta-ctfB-deficient C. acetobutylicum CEKW, in which genes encoding phosphotransacetylase and CoA-transferase were knocked-out, was assessed for its potential as a butyric acid-producer in fermentations with four controlled pH-values at 5.0, 5.5, 6.0, and 6.4. The CEKW strain was further engineered by knocking-out the adhE1 encoding aldehyde/alcohol dehydrogenase to prevent solvent-production. The simultaneous deletion of the pta-ctfB-adhE1 resulted in metabolic switch from biphasic to acidogenic fermentation enhancing butyric acid-production. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556).]
저자 천승우, 장유신, 이상엽
소속 KAIST
키워드 Clostridium acetobutylicum; Metabolic Engineering; Butyric Acid
E-Mail nall001@kaist.ac.kr
원문파일 초록 보기