| 초록 |
The main objective in gene therapy is the development of efficient, non-toxic gene carriers that can transport DNA inside the cell nucleus of the target cell. Nonviral vectors have gained a great deal of interest by their advantages of increased safety, reduced immunogenicity and ease of manufacture compared to viral vectors. The key steps of in vitro nonviral vector gene delivery have been identified as : condensation of DNA into compact particles, uptake of the complexes by the target cells through endocytosis, and transport into the nucleus. We designed monomers to produce cationic poly(carbonate)s that have protonable primary amine group to condense DNA, secondary or tertiary amine group to provide endosomal buffering effect and biodegradable carbonate backbone linkage. Controls in polymer molecular weight were obtained by ring-opening anionic polymerization. We focused on the effect of polymer molecular weight, degradation rate, and density of cationic charges on cytotoxicity and transfection efficiency. |
