| 초록 |
Gold nanoparticles (AuNPs) formed on a glassy carbon (GC) electrode was used to prepare a tyrosinase enzyme electrode. Soluble gold (AuCl4-) was reduced to nanoparticles by electrodeposition method while the applying potential and time were optimized through the double-pulse technique to control the coverage of nanoparticles. On the AuNP deposited electrode, tyrosinase (TYR) was immobilized by the formation of self-assembled monolayer (SAM) using 3-mercaptopropionic acid (MPA) and covalent binding. The prepared tyrosinase electrode was compared with bare, AuNP-, and MPA-AuNP-GC electrodes in terms of cyclic voltammetry. The voltammograms well represent the enzymatic oxidation of catechol and phenol. Further, the prepared TYR-AuNP-GC electrode performed reasonably to measure concentration of a pesticide, 2,4-dichlorophenoxyacetic acid (2,4-D) by inhibition of tyrosinase activity in oxidation of catechol. |
