| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.447 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Tissue engineering spinal cord using bone marrow stromal stem cells seedking polymeric scaffolds |
| 초록 | Bone marrow stromal stem cells (BMSCs) are a source of stem-like and progenitor cells. BMSCs transplanted into the normal animal central nervous systems are able to differentiate into neural cells. The transplantation of adult BMSCs directly into adult rat brain reduced functional deficits associated with stoke. The mesenchymal cells of bone marrow express neural phenotype and migrate when placed in damaged brain. In addition, nerve growth factor (NGF, BDNF) appear to enhance the regrowth of ascending sensory axon across spinal cord gap in adult rats. Also, porous scaffolds have highly porous structure with good interconnection between pores for allowing cell adhesion and growth. We postulated that resected segment of the spinal cord could be regenerated in adult rats gap implanting BMSCs and BDNF associated with polymeric porous scaffold. Fabrication of nerve guidance channeled poly(L-lactide –co-glycolide) (75:25 by mole ratio of lactide to glycolide, PLGA) scaffold performed by ice-particle leaching method. It investigated as potential guidance channel. BMSDs were harvested from the femurs and tibias of adult female Fischer rats. BMSCs were suspended at 2x106 cells/scaffold in PLGA scaffold with 200 ng BDNF. Fischer rats with the gap (T8-T9 : 5 mm, 3 mm ,1 mm and Cutting) created through the spinal cord resection were implanted by the PLGA scaffold consisting of BMSCs and BDNF. For histological evaluation, the implants were removed after 4 and 8 weeks. Thin sections were cut from paraffin embedded tissue and histological section were stained by hematoxylin and eosin (H&E) staining and immunohistochemical staining for neurofilament (NF), neuro-specific enolase (NSE) and S-100 protein Motor functional outcome measurements using the Basso-Beattie-Bresnehan (BBB) score performed weekly up to 8 weeks post-injury. Survival of Fischer rats with designed gap observed up to 8 weeks after implantation of spinal cord transaction. Survival of rats in blank control group was bad due to bladder burst and ulcer. Survival of G3 was better than G2. Also, survival of G3-3 was better than G3-5. The BBB locomotor rating score measured in the four groups up to 8 weeks after implantation of spinal cord transaction. Histological examination confirmed the presence of neural tissue in G3 rats, which consisted mostly of unorganized tissue with bundles of cells that appeared to be axons. Also, NR-positive areas were observed in the regenerated tissue of G3 although the laminar organization observed in the native cord was lost. Acknowledgement This study was supported by KMOICE (N11-A08-1402-05-1-3) References 1. MP Vacanti, JL Leonard, B Kore, et al. Transplan. Proceed., 33. 592 (2001) 2. D Woodbury, EJ Schwarz, DJ Prockop et al., J. Neurosci. Res., 61, 364 (2000) |
| 저자 | 김선경1, 홍금덕2, 김순희2, 김연식2, 김문석1, 강길선2, 이해방1 |
| 소속 | 1한국화학(연), 2전북대 |
| 키워드 | Spinal cord; Bone marrow stromal stem cell; BDNF; PLGA |
