| 학회 |
한국재료학회 |
| 학술대회 |
2011년 봄 (05/26 ~ 05/27, 제주 휘닉스 아일랜드) |
| 권호 |
17권 1호 |
| 발표분야 |
D. Health and Biological Materials(생체 및 의용재료) |
| 제목 |
Optimization of Corsslinked Polyvinyl Alcohol-Gelatin Hydrogels to Obtain High Mechanical Properties and Excellent Biocompatibility for Bone Regeneration |
| 초록 |
The aim of this investigation was to optimize crosslinking conditions of polyvinyl alcohol/gelatin hydrogels using genipin as naturally crosslinker for bone application. To satisfy implantation, compressive strength, materials characterization, biocompatibility and biodegradation were investigated. Ratios of polyvinyl alcohol/gelatin:genipin (1:1) offered excellent compressive strength (wet: 8 MPa, lyophilized: 20 MPa) among series ratios. Crosslinking reaction of gelatin and genipin during synthesis process was reflected by fourier transform spectroscopy. Crosslinked hydrogels were non-toxic with over 90 % cell viability in 100 % extract solution using MTT testing. Biocompatibility testes showed excellent cell attachment and cell proliferation using osteoblast cells (MG-63), chondrocytes cell and mesenchymal stem cells (MSC). Protein expression of beta-actin, osteoglycine and PCNA showed that the crosslinked hydrogels were non-harmful for cell membrane and nucleus using western blot testing. Crosslinked hydrogels started to degrade at day 10th and totally degraded after 3 month in which satisfy to shorten healing process. |
| 저자 |
Thi-Hiep Nguyen1, Shin-Woo Kim2, Young-Ki Min3, Hun-Mo Yang4, Byong-Taek Lee5
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| 소속 |
1Department of Biomedical Engineering and Materials, 2College of Medicine, 3Soonchunhyang Univ., 4Cheonan, 5330-090 |
| 키워드 |
Polyvinyl alcohol; gelatin; genipin; hydrogels
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| E-Mail |
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