| 초록 |
A chemical absorption technology using amine aqueous solution to capture CO2 causes by-product due to harsh condition process. It is an effective eco-friendly way to replace the amine solution with biocatalyst. But the CO2 sequestration process is worked at harsh condition We found Hahella chejuensis carbonic anhydrase (HCCA) derived from domestic marine bacterium having high pH stability. To adjust CO2 sequestration system, long time thermal stability and recyclable are necessary. We performed HCCA immobilization system based on silicification of a monosilicate, tetramethyl orthosilicate (TMOS), and spermine derived from organic compound having 4 amine group. Free HCCA lost their activity in 1 hour at 60 degree, in contrast, immobilzed HCCA by silica particle endured over 2hour with 80% activity. In 13 times recycling test, immobilized HCCA activity was maintained 80%. Thus monosilicate immobilization of HCCA is ready to adjust CO2 sequestration system. |
