| 학회 |
한국고분자학회 |
| 학술대회 |
2002년 가을 (10/11 ~ 10/12, 군산대학교) |
| 권호 |
27권 2호, p.318 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
RELEASE OF NEWCASTLE DISEASE VIRUS VACCINE FROM CHITOSAN MICROSPHERE IN VITRO AND IN VIVO |
| 초록 |
A new and improved method of vaccine delivery is urgently needed to decrease mortality due to infectious diseases. One possible way of facilitating this work is to reduce the number of repeated administrations required for long-term protection. Although the introduction of an efficient oral vaccine would diminish costs, patient discomfort and the need for qualified personnel to administer the vaccine, most vaccines still have to be administered by injection. Microcapsules or microspheres were the most common forms used for the controlled-vaccine delivery formulations. Newcastle disease vaccine (NDV)-loaded chitosan microspheres (NDV-CM) were prepared. Stimulatory effects of these NDV-CM on antibody response compared to free NDV were examined in vitro and in vivo. In vitro stimulation of macrophages with virus vaccine resulted in higher number of cells compared to saline-treated control. Both NDV and NDV-CM induced secretion of IL-1 in dose dependent manner and the secretion of IL-1 by NDV-CM was delayed compared to free NDV. In case of NDV whole virion contrary to NDV subunit, chitosan microspheres loaded with NDV whole virion had the similar antibody responses and protection of chickens with the commercial ND-K as a control, indicating that all of vaccinated chickens survived after challenge. |
| 저자 |
박인규1, 윤철희2, 최윤재3, 김선중*4, T. Akaike**5, 조종수6
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| 소속 |
1서울대, 2*서울대, 3**Department of Biomolecular Engineering, 4Tokyo Institute of Technology, 5Yokohama, 6226-8501 |
| 키워드 |
Newcastle vaccine; microsphere; chitosan; immunization; immunotherapy
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| E-Mail |
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