| 초록 |
Cancer treatments have improved since the advancement of cancer immunotherapy. However, limitations for the clinical utilities have still existed. Here, to overcome previous immunotherapeutic strategies, we prepared the dendritic cell (DC) mimicked nanomaterials, named artificial nanoDCs (anDCs), through nanocellularization. Using this tool, we could functionalize nanoparticles with DC membrane and also optimized the highest therapeutic efficient anDCs by varying membrane/particle area fractions. The optimized anDCs revealed strong stability even after prolonged storage and have tumor suppression potential similar to conventional DCs. Finally, to maximize tumor suppression of anDCs, aCTLA-4 was engineered to the anDCs. As a result, aCTLA-4 conjugated anDCs could boost immune response and showed dramatic anticancer efficacy. Thus, the anDCs can potentially be applied for next-generation cancer immunotherapy drugs in a combination of functionalization of various immune-boosting agents. |
