| 초록 |
The effects of mechanical tension on cellular metabolic responses were investigated experimentally using a bioreactor. The effects of various chemical factors, such as growth factors, cytokines, and hormones, on cellular metabolic responses were also investigated. Based on previous reports demonstrating the important roles of mechanical factors in the growth and activity of MSCs, we sought to evaluate the effects of mechanical stimuli on the proliferation of bone marrow-derived MSCs using a cell training bioreactor that imposed cyclic mechanical stretch with parameters of 240 min/day, 0.03 Hz, 5-15% strain. The application of various cyclic stretch to MSCs enhanced their proliferation during the early stage but not during the late stage of batch culture. Mechanical stretch did not increase the release of LDH from MSCs during culture. Appropriate levels of mechanical tension increased collagen synthesis but did not alter MSC surface antigen expression. It is thought that the appropriate level of mechanical tension served as a potent positive modulator of MSC proliferation during the initial stages of culture. |
