| 초록 |
Three-dimensional (3-D) bioprinting has been widely used to construct functional biological structures useful in many biomedical applications, including tissue engineering appraoches. We previously reported that oxidized hyaluronate (OHA) and glycol chitosan (GC) could form self-healing hydrogel in the presence of adipic acid dihydrazide (ADH). In addition, the mechanical stiffness and stability of OHA/GC/ADH hydrogel could be enhanced when a small amount of alginate (ALG) was added to the gel in the presence of calcium ions for secondary cross-linking. In this study, hyaluronate-alginate hybrid (HAH) polymer was synthesized and added to OHA/GC/ADH hydrogel to fabricate stable but self-healing hydrogel for 3-D bioprinting. Various characteristics of OHA/GC/ADH/HAH hydrogel including mechanical stiffness, self-healing efficiency and cell viability were investigated. |
