| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터) |
| 권호 |
43권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Enhanced fluorescent signal of a TNT-sensing microbead by controlling surface charge |
| 초록 |
Poly (2-hydroxyethyl methacrylate)(poly(HEMA))-based hydrogle beads were used as a platform for microbial-based biosensor for detection to trinitrotoluene(TNT) by loading a green fluorescent protein(GFP)-producing Escherichia coli(E. coli). In order to enhance cell affinity for performance of the sensor, 2-(methacryloyloxy) ethyl trimethylammonium chloride(MAETC) as a cationic monomer was modified using different ratio of MAETC to HEMA. The poly(HEMA-MAETC) hydrogel beads were successfully fabricated via a novel electro-spray using photo-polymerization method in one-step. These charge controlled beads by MAETC composition indicated 9 times improved cell affinity by electrical interaction between charged beads and bacteria compared to that of poly(HEMA). As a result, maximum 5 times enhanced fluorescence intensity was observed at the poly(HEMA-MAETC)(70mol% MAETC) bead compared with poly(HEMA) bead after exposure to TNT, indicating enhanced performance as a biosensor to TNT. |
| 저자 |
김수현1, 김현지1, 천교1, 차채녕2, 이강석2, 이성국2, 노현지2, 김영균1, 이원목3, 이현정1
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| 소속 |
1국민대, 2울산과학기술원, 3세종대 |
| 키워드 |
Poly (HEMA-MAETC) beads; Charge-controlled micro beads; Electro-spraying method; Photo-polymerization; Biosensor for TNT-sensing
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| E-Mail |
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