| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Intervertebral Disc Regeneration using SIS Scaffolds |
| 초록 | Objective: Intervertebral disc degeneration causes back pain and disability. A solution of the problem developed using biomaterials such as polymer and ceramic scaffold. Tissue engineering technique using biodegradable polymer, cytokine and cell might be supported very effective and viable organ. Small intestinal submucosa (SIS), which was natural materials improvement attachment, proliferation and differentiation. In study, we experimented 3D of disc cell of using SIS scaffold. Method: We manufactured SIS 1％, EDC 100mM sponge for using scaffold, and cultured disc cell from rabbit was seeded 1x105 cells/scaffold concentration. Then it designed Four groups such as (1) monolayer cultured group in growth media and chondrogenic media included TGF-β1 as positive control (2) cultured group growth media in SIS scaffold, and (3) in chondrogenic media TGF-β1 in SIS scaffold. (4)cultured group growth media in PLGA scaffold as negative control. After seeded cell, we observed charcterization of disc cell by MTT assay, RT-PCR on 3, 7, 14 and 21 day. Result: In MTT result, we observed that chondrogenic media better than growth media for cell proliferation. RT-PCR results, we confirmed more express type Ⅱ collgen and aggrecan in chondrogenic media. Conclusion: when we cultured disc cell in vitro, we observed better to keep the characteristic of disc cell in 3D than that in 2D culture, and more stable to culture disc cell in chondrogenic media than that in growth media. Acknowledgement: This work was supported Musculoskeletal Bioorgan Center (0405-BO01-0204-0006) by KMOHW. References: 1. C. A. Seguin, M. D. Crynpas, R. M. Pilliar, et. al., Spine, 29, 1299 (2004) 2. M. Takigawa, E. Shirai, K. Fukuo, K. Tajima, et. al., Bone and Mineral, 2, 449 (1987) |
| 저자 | 하현정1, 박기숙1, 최방실1, 김순희1, 윤선중1, 김문석2, 강길선1, 이해방2, 이종문1 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | disc cell; SIS; tissue engineering |
