초록 |
Hydrogen production by microalgae is an environmentally feasible strategy of reducing CO2, but the productivity of microalgae-based systems is still limited and needs to be improved, particularly under autotrophic conditions in which starch metabolism is crucial for hydrogen fermentation in microalgae. During autotrophic H2 production phase, the starch is the only carbon source to play a significant role in respiratory consumption of oxygen for maintaining anaerobic conditions in photobioreactor. Herein, we investigated the effect of inducing a CO2 concentrating mechanism (CCM) on the induction phase of autotrophic starch accumulation and hydrogen production in C. reinhardtii. CCM-induced Chlamydomonas cells showed a highly efficient synthesis and degradation of starch due to its increased photosynthetic ability during sulfur deprivation, thereby enhancing autotrophic H2 production. The low-CO2-mediated induction prior to sulfur deprivation is a cost-effective strategy for enhancing photoautotrophic H2 production using microalgae. |